Synthesis, Subcloning, and Multi-Fragment Assembly

•Generation of fragments sourced from de novo synthesis, your existing inventory, or ours, with host-specific codon optimization where needed.
•Assembly or subcloning of one or more fragments into new or existing backbones
•Selection and design of assembly strategy

Backbone Engineering

•Customization of the vector backbone, independent of or alongside insert work. •Expression tuning: promoter, UTR, and regulatory element selection for your target host and cell type.
•Propagation and selection: Origin and selection marker swaps for bacterial propagation and downstream workflows.

Complex Constructs

•Plasmids with inherent assembly challenges: viral vectors (AAV with ITR-flanked cassettes, lentiviral, adenoviral), mRNA and IVT templates (homopolymers), repetitive elements, large inserts, and high-GC regions.